| Chapter | University | Type | Area | Founded |
| Alpha Omicron | Johnson C. Smith University | College Chapter | Area 3055 | 5/4/1923 |
| Kappa Lambda | Greensboro, NC | Alumni Chapter | Area 3054 | 6/9/1923 |
| Phi Lambda | Raleigh, NC | Alumni Chapter | Area 3053 | 12/19/1926 |
| Beta Epsilon | North Carolina A&T State University | College Chapter | Area 3054 | 3/28/1929 |
| Alpha Pi Lambda | Winston-Salem, NC | Alumni Chapter | Area 3054 | 6/1/1931 |
| Beta Rho | Shaw University | College Chapter | Area 3053 | 10/31/1936 |
| Beta Theta Lambda | Durham, NC | Alumni Chapter | Area 3053 | 5/7/1938 |
| Beta Nu Lambda | Charlotte, NC | Alumni Chapter | Area 3055 | 8/1/1939 |
| Beta Mu Lambda | Salisbury, NC | Alumni Chapter | Area 3055 | 8/21/1939 |
| Gamma Beta | North Carolina Central University | College Chapter | Area 3053 | 5/23/1941 |
| Gamma Kappa Lambda | Wilmington, NC | Alumni Chapter | Area 3052 | 1/1/1945 |
| Gamma Mu | Livingstone College | College Chapter | Area 3055 | 2/27/1947 |
| Gamma Psi | Saint Augustine’s College | College Chapter | Area 3053 | 3/15/1948 |
| Beta Iota | Winston-Salem State University | College Chapter | Area 3054 | 4/28/1951 |
| Epsilon Rho Lambda | Fayetteville, NC | Alumni Chapter | Area 3052 | 1/1/1952 |
| Beta Zeta | Elizabeth City State University | College Chapter | Area 3051 | 2/21/1953 |
| Epsilon Chi Lambda | Elizabeth City, NC | Alumni Chapter | Area 3051 | 12/7/1953 |
| Zeta Eta Lambda | Greenville, NC | Alumni Chapter | Area 3051 | 1/1/1955 |
| Epsilon Zeta | Fayetteville State University | College Chapter | Area 3052 | 11/3/1958 |
| Eta Mu Lambda | Gastonia, NC | Alumni Chapter | Area 3055 | 1/1/1960 |
| Theta Omicron Lambda | Goldsboro, NC | Alumni Chapter | Area 3052 | 1/1/1964 |
| Eta Nu | East Carolina University | College Chapter | Area 3051 | 4/3/1971 |
| Eta Omicron | North Carolina State University | College Chapter | Area 3053 | 4/7/1971 |
| Kappa Omicron | Duke University | College Chapter | Area 3053 | 12/7/1975 |
| Mu Zeta | University of North Carolina at Chapel Hill | College Chapter | Area 3053 | 4/15/1976 |
| Mu Tau | University of North Carolina at Charlotte | College Chapter | Area 3055 | 1/1/1977 |
| Epsilon Sigma Lambda | Rocky Mount, NC | Alumni Chapter | Area 3051 | 2/5/1977 |
| Nu Zeta | Western Carolina University | College Chapter | Area 3055 | 5/7/1977 |
| Xi Eta | Wake Forest University | College Chapter | Area 3054 | 5/4/1979 |
| Nu Iota Lambda | Kinston, NC | Alumni Chapter | Area 3051 | 12/5/1979 |
| Omicron Beta | Wesleyan University | College Chapter | Area 3051 | 10/9/1980 |
| Omicron Theta | University of NC at Wilmington | College Chapter | Area 3052 | 5/5/1981 |
| Pi Zeta | University of NC at Greensboro | College Chapter | Area 3054 | 10/7/1984 |
| Pi Nu | Appalachian State University | College Chapter | Area 3055 | 1/17/1985 |
| Sigma Delta | Elon University | College Chapter | Area 3054 | 9/29/1991 |
| Rho Beta Lambda | Henderson, NC | Alumni Chapter | Area 3053 | 1/25/1998 |
| Rho Alpha Lambda | Jacksonville, NC | Alumni Chapter | Area 3052 | 3/28/1998 |
| Rho Theta Lambda | Ahoskie, NC | Alumni Chapter | Area 3051 | 6/4/2000 |
| Tau Nu | University of North Carolina at Pembroke | College Chapter | Area 3052 | 1/23/2001 |
| Tau Omicron | Davidson College | College Chapter | Area 3055 | 11/1/2003 |
| Upsilon Iota | Chowan University | College Chapter | Area 3051 | 6/25/2011 |
| Phi Gamma | Methodist University | College Chapter | Area 3052 | 7/14/2017 |
| Phi Delta | Johnson & Wales University | College Chapter | Area 3055 | 7/14/2017 |
| Phi Pi | High Point University | College Chapter | Area 3054 | 03/22/2022 |
